ASTM E1674-99(2003)

1.1 This test method covers an inhalation procedure to investigate the cardiac sensitization of volatile chlorinated hydrocarbons and other volatile solvents.

1.2 This test method is primarily a screening tool. The procedure does permit a rank order of the sensitization potential of the compounds tested, but is not recommended for establishing significant effect levels.

1.3 This test method assumes that the user is knowledgeable in mammalian toxicology, electrocardiography with animals and other pertinent areas, and relies heavily on the judgment of the investigator.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

This test method is designed to provide information as to any dose level of test material that gives rise to clear signs of compound-induced cardiac sensitization.

Cardiac sensitization has occurred in humans during periods of stress while being exposed to high concentrations of some unsubstituted and halogenated hydrocarbons.³

The dog is employed for this study because the conclusions from this test method can be applied to man with much more certainty than those from other animals.⁴