DIN EN 12821:2009-08

This European Standard specifies a method for the determination of vitamin D in foodstuffs by high performance liquid chromatography (HPLC). In the majority of foodstuffs vitamin D is naturally present as cholecalciferol, vitamin D3, and this is the form of the vitamin determined. Vitamin D2, ergocalciferol, is sometimes present in fortified foodstuffs and can also be determined using this European Standard. Some foods will contain both vitamin D3 and D2. This European Standard is not applicable to these samples. This European Standard provides the base for the analytical methods. It is intended to serve as a frame in which the analyst can define his own analytical work in accordance to the standard procedure.