DIN EN 17252:2018-06

This European Standard describes a procedure based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) for the determination of phomopsins in lupin seeds and lupin-derived products. Several phomopsins exist, i.e. phomopsin A, B, C and D, but the method only deals with the quantitative measurement of phomopsin A due to lack of commercially available analytical reference standards for the other phomopsins.The method has been validated for phomopsin A in naturally contaminated lupin seeds, lupin flour and a bakery product (crisp bread) at levels ranging from approximately 5 µg/kg to 60 µg/kg.