DIN EN 17254:2018-05

Celiac disease (CD) is a life-long autoimmune disease of the small intestine, primarily affecting genetically susceptible individuals. Its prevalence has been estimated to be 1% of the population worldwide. CD becomes manifest in a chronic enteropathy, caused by an irreversible intolerance for gluten. CD, if untreated, is associated with increased morbidity and the only accepted treatment is a strict and lifelong adherence to a gluten free diet, which interrupts the immune response triggered by gluten.The gluten concentration in food samples can be measured by different test methods. Although the use of mass spectrometry is possible, the most commonly used technique is the enzyme-linked immunosorbent assay (ELISA). This document specifies minimum method performance requirements for enzyme-linked immunosorbent assays that quantify non-fragmented or fragmented gluten from wheat (e. g. Triticum aestivum), rye, and barley in raw and processed foodstuffs.