DIN EN ISO 15753:2016-11

This standard describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: - a general method; - a specific method for coconut oil and vegetable oils with short-chain fatty acids. These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this International Standard. The polycyclic aromatic hydrocarbons are extracted with an acetonitrile/acetone mixture followed by purification on C18 reversed-phase and then Florisil bonded-phase catridges. Determination of the content of the individual polycyclic aromatic hydrocarbons after separation is achieved by means of high-pressure liquid chromatography (HPLC) and by measuring the fluorescence at various excitation and emission wavelengths.