ISO 20397-3:2025

This document specifies general requirements and guidance for metagenomics-dedicated sample preparation, generating and analyzing metagenomics sequence data obtained from massive parallel sequencing platforms. The specified metagenomics process includes the following stages: a) Sampling strategy and process, including type, storage, transportation, extraction, quality; b) Nucleic acid library preparation c) Sequencing strategy and assessment; d) Database construction; e) Bioinformatics analysis and report f) Validation and evaluation for bioinformatics pipeline, and database. This document applies to laboratories and research organizations.