NBN ISO 23443:2021

This document specifies a method for the quantitative determination of ß-carotene and lycopene in
infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid
concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)
and UV-visible detection. The application range runs from 1 µg/100 g to 1 500 µg/100 g for lycopene
and from 1 µg/100 g to 2 250 µg/100 g for ß-carotene. Based on the single-laboratory validation, the
limit of detection (LOD) was 0,1 µg/100 g and the limit of quantification (LOQ) was 0,3 µg/100 g for
each carotenoid.
The method does not apply to materials that contain measurable levels of ß-apo-8'-carotenal. The
reproducibility data meets the requirements given in References [8] and [10].
Annex C specifies the determination of lutein. The reproducibility data does not meet the requirements
given in Reference [9].