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NBN ISO/TS 21569-2:2021
Molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products
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Summary
This document specifies a procedure for the detection of a DNA sequence present in a genetically
modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose,
extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by
amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase
gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1)
from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also
be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The
application of this method requires the extraction of an adequate amount of amplifiable DNA from the
relevant matrix for the purpose of analysis.
modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose,
extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by
amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase
gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1)
from a Class 1 integron of Escherichia coli.
The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also
be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The
application of this method requires the extraction of an adequate amount of amplifiable DNA from the
relevant matrix for the purpose of analysis.
Technical characteristics
| Publisher | Bureau de Normalisation Belge (NBN) |
| Publication Date | 10/22/2021 |
| Page Count | 20 |
| EAN | --- |
| ISBN | --- |
| Weight (in grams) | --- |
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