XP ISO/TS 4985, V04-201 (11/2023)

This document specifies a microplate fluorometric method for the determination of alkaline phosphatase activity (ALP, EC 3.1.3.1)[5] in raw and heat-treated whole, semi-skimmed, skimmed milk, in cream, flavored milks and cheeses. The method is applicable to milk and beverages made from cow's, sheep's and goat's milk. Although the method has not been tested on the milk of other species, it can also be applied to the milk of species whose composition is similar to that of cow, sheep or goat milk, such as buffalo or camelid milk. It is also applicable to milk powder after reconstitution and to soft, uncooked pressed and cooked pressed cheeses, provided that the mold is only present on the surface of the cheese and has not spread to the cheese. inside, as in the case of blue-veined cheeses, for example. For large cooked pressed cheeses, specific sampling conditions apply (see Article 7). NOTE This method was adapted from Reference [6].
(Automatic translation from French)